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43746-G2
Controls on the Hydrogen Isotopic Composition of Petroleum Hydrocarbons
Alex L. Sessions, California Institute of Technology
The goal of this project has been to understand the process of hydrogen
isotopic exchange in organic molecules from sedimentary environments. Such
processes affect even C-bound H over million-year timescales at temperatures
above ~80°C, and thus potentially impact the D/H ratios of petroleum
hydrocarbons. Because this exchange is so slow, laboratory experiments on
simple hydrocarbons are not feasible even at elevated temperatures. Our
approach has been to combine laboratory incubation experiments of labile molecules
with ab initio density functional theory (DFT) calculations
for more recalcitrant hydrocarbons. We have conducted exchange experiments on
simple ketones, taking advantage of rapid keto-enol tautomerism under base
catalysis to achieve equilibration of the adjacent (a) hydrogens over several days. The results of
these experiments then provide a calibration dataset for DFT estimates of vibrational frequencies, which can be used to directly
calculate equilibrium D/H fractionations for hydrocarbon moieties of choice.
Experimental incubations were conducted for 7 ketone
substrates, included both linear and cyclic compounds at temperatures from 25
to 70°C. The experimental data yield equilibrium fractionation factors ranging
from 0.802 (cyclohexanone at 70°C) to 1.027 (2,4-dimethyl-3-pentanone
at 25°C), with fractionations increasing in the order: cyclic 2° < linear 1°
< linear 2° < linear 3° (Figure 1). Experimental and DFT fractionations
are in excellent agreement for these compounds, with a correlation coefficient
(R2) of 0.979. Estimates
of fractionations for hydrocarbon H based on DFT calculations are shown in Figure
2. In general, we predict that n-alkanes
will be depleted in D relative to water at equilibrium by 80 to 100‰,
regardless of temperature. The very weak temperature dependence was unexpected,
and derives from the opposite temperature dependence of effects at 1° versus 3°
positions. This dataset now provides a quantitative basis for interpreting
organic D/H ratios in mature sediments, oils, and gases. More specifically, it
will be possible to assess whether differing organic structures are in isotopic
equilibrium and thus have been subject to extensive hydrogen exchange, or
whether they potentially preserve original environmental signals. A manuscript
describing these results is currently in preparation.
One additional outcome of this project has been a more complete
understanding of processes affecting accuracy and precision in GC-pyrolyis-IRMS. Because our experiments require very
accurate measurement of organic D/H over a large range, we synthesized multiple
organic standards having dD values ranging from -200 to +800‰. Careful
manipulation of the order and D/H ratio of these standards allows us to show
that there is a small, systematic memory effect in GC-pyr-IRMS
systems, such that 2-4% of the hydrogen in each chromatographic peak is derived
from that in the preceding peak. In experiments such as ours, where the desired
results are obtained by varying D/H ratios over hundreds of permil,
this memory can introduce a systematic bias of 20-40‰ in fractionation factors unless
properly accounted for. A manuscript describing these effects is currently in
review by Analytical Chemistry.
| | | |
| Figure 1. Experimental and DFT estimates of al/w for 1° (solid line), 2° (dashed), and 3° (dotted) hydrogens in linear ketones. Symbols are experimental data. | |
| | Figure 2. DFT estimates of al/w in hydrocarbons. Black lines are as for Fig 1. Gray lines represent estimates for n-alkanes (light gray) and isoprenoids (dark gray). | |
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